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目的建立一种膜片钳技术(patchclamptechnique,PCT)的成年大鼠海马CAI区锥体细胞(adultrathippocampalCAIpyramidalneuron,ARHCPN)的急性分离方法。方法采用酶加机械分离的方法制备ARHCPN,用相差显微镜观察其形态特征,并用PCT的细胞贴附模式记录L-型Ca2+通道的单通道电话动。结果所分离的ARHCPN不仅形成学特征保存完好,而且保存了L型Ca2+通道的电生理学特性。结论成功地建立了一种简单、快速、可靠的适用于膜片钳单通道记录的ARHCPN的急性分离方法。
OBJECTIVE: To establish an acute isolation method of adult rat hippocampal CAI pyramidal cells (ARHCPN) in a rat model of patchclamptechnique (PCT). Methods ARHCPN was prepared by enzymatic and mechanical separation. Morphological characteristics of ARHCPN were observed by phase contrast microscopy, and single-channel electroporation of L-type Ca2 + channel was recorded by PCT cell attachment mode. Results The isolated ARHCPN not only formed a well-preserved characteristic, but also preserved the electrophysiological properties of L-type Ca2 + channels. Conclusion A simple, rapid and reliable ARHCPN method for acute isolation of single-channel patch-clamp recordings was successfully established.