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用30只3～35天龄的新生小鼠,对其母鼠于妊娠18天至产后30～35天的哺乳期,每日通过胃管灌注50mg丙基硫氧嘧啶,使新生小鼠甲状腺功能过低。对12只正常小鼠于生后3、6、9、12、14及21天进行检查作为对照。对生后21、28及35天的甲状腺机能低下小鼠还作电生理检查,看耳蜗电位是否已消失。处死后对其耳蜗进行光镜、透射电镜、部分并用扫描电镜观察。发现甲状腺机能低下的小鼠螺旋器有严重异常,Kolliker′s器(为胚胎发育过程中位于内沟区的上皮结构)处于极不成熟阶段,且迟不消失,盖膜明显变形,感觉上皮细胞发育不全;到出生后第35天,螺旋器隧道仍未开放,感觉毛细胞和支持细胞显不成熟状,并异常地保有动纤毛。作者们从而认为,这类小鼠的听力丧失可从电镜下找到形
Thirty newborn mice aged 3 ~ 35 days were used to inject 50mg propylthiouracil per day through gastric tube during their lactation from 18 days gestation to 30-35 days postpartum. The thyroid function of neonatal mice Too low. 12 normal mice were examined at 3, 6, 9, 12, 14 and 21 days after birth as a control. The hypothyroid mice at 21, 28 and 35 days after birth were also subjected to electrophysiological examination to see if the cochlear potential had disappeared. After the death of the cochlea light microscope, transmission electron microscopy, and observed by scanning electron microscopy. It was found that hypothyroid mice spiral serious abnormalities, Kolliker’s device (for the embryonic development in the inner groove region of the epithelial structure) is in a very immature stage, and disappear, the apparent deformation of the membrane, the sensory epithelial cells Hypoplasia; to the 35th day after birth, the spiral tunnel remains unopened, sensory hair cells and supporting cells appear immature, and abnormally retained moving cilia. The authors thus believe that hearing loss in these mice can be found under electron microscopy