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目的 观察中药脑肺康对缺氧性肺动脉高压和肺气肿的防治效果并探讨其作用机制。方法 以弹性蛋白酶溶液滴注入金黄地鼠气管内 ,正常饲养 30 d后 ,常压下缺氧 15 d,分别设立中药脑肺康预防组、治疗组、肺气肿 +缺氧组及正常对照组。于处死动物前 ,测量平均肺动脉压 ,取静脉血进行循环内皮细胞计数 ;处死后测量右心肥大指数 ,光镜下计数左侧肺支气管肺泡灌洗液内细胞总数 ,对右侧肺进行光镜图像分析。结果 中药脑肺康预防组和治疗 3个月组平均肺动脉压、循环内皮细胞数、支气管肺泡灌洗液细胞总数、肺泡平均内衬间隔和肺细小动脉平均中膜面积百分比均明显低于肺气肿 +缺氧组 (P<0 .0 5或 P<0 .0 1)。结论 中药脑肺康可降低肺动脉压 ,保护血管内皮细胞及减轻肺内炎症反应 ,减缓肺气肿病变的发展 ,减轻缺氧造成的肺血管重建。
Objective To observe the preventive and therapeutic effects of Naofeikang on hypoxic pulmonary hypertension and emphysema and explore its mechanism. Methods The elastase solution was injected into the trachea of golden hamsters. After normal feeding for 30 days, the rats were anoxic for 15 days under normal pressure. The Chinese medicine Naofeikang preventive group, treatment group, emphysema plus hypoxia group and normal control were established respectively. group. Before the animal was sacrificed, the mean pulmonary artery pressure was measured, and the venous blood was taken for circulating endothelial cell count; the right heart hypertrophy index was measured after the death; the total number of cells in the left lung bronchoalveolar lavage fluid was counted by light microscopy, and the right lung was subjected to light microscopy. Image analysis. Results The mean pulmonary arterial pressure, the number of circulating endothelial cells, the total number of cells in the bronchoalveolar lavage fluid, the interval between the average alveolar lining, and the percentage of the mean medial area of the pulmonary fine arteries in the NFL group and the 3-month group were significantly lower than those in the lung Swollen+hypoxia group (P<0.05 or P<0.01). Conclusion Chinese medicine Naofeikang can reduce the pulmonary artery pressure, protect the vascular endothelial cells and reduce the inflammatory reaction in the lung, slow down the development of emphysematous lesions, and alleviate the pulmonary vascular reconstruction caused by hypoxia.