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针对多种食品基质(葡萄酒、汽水、黄酒、植物蛋白饮料、乳饮料、花生酱、芝麻酱和糕点)建立了同时测定赤藓红、亮蓝、诱惑红、日落黄、靛蓝、柠檬黄、苏丹红Ⅰ、苏丹红Ⅱ、苏丹红Ⅲ、苏丹红Ⅳ、酸性红1、酸性红4、酸性红8、酸性红14、酸性红88和酸性红97这16种合成着色剂的高效液相色谱-质谱联用分析方法。根据样品种类和所含成分的差别,建立了不同的样品预处理方法,并且通过对提取溶剂的讨论,采用混合溶剂V(甲醇)∶V(乙腈)=2∶3作为提取试剂,比单一溶剂的提取效率更高。萃取液经Kromasil 100-5型色谱柱(150 mm×2.1 mm×5.0μm)分离,外标法定量。结果表明,16种合成着色剂呈现良好的线性关系(r~2=0.998 9~0.999 8),方法的检测限为0.10或0.010 mg/kg,进行低、中、高3个浓度水平添加实验的平均回收率为86.5%~97.3%,相对标准偏差<4.9%(n=6)。该方法重现性好,适合于多种食品中上述16种合成着色剂的检测。
A method was developed for the simultaneous determination of erythrosine, brilliant blue, seductive red, sunset yellow, indigo, lemon yellow, Sudan I, arsenic trioxide and so on for a variety of food matrices (wine, soft drinks, rice wine, vegetable protein drinks, dairy drinks, peanut butter, sesame and cakes) , Sudan II, Sudan III, Sudan IV, Acid Red 1, Acid Red 4, Acid Red 8, Acid Red 14, Acid Red 88 and Acid Red 97 were analyzed by high performance liquid chromatography-mass spectrometry Use analytical methods. According to the differences of sample types and contained components, different sample pretreatment methods were established, and the solvent V (methanol): V (acetonitrile) = 2: 3 was used as extraction reagent, The extraction efficiency is higher. The extract was separated on a Kromasil 100-5 column (150 mm × 2.1 mm × 5.0 μm) and quantified by external standard method. The results showed that the 16 synthetic colorants showed a good linearity (r ~ 2 = 0.998 9 ~ 0.999 8). The detection limit of this method was 0.10 or 0.010 mg / kg. The average recoveries were 86.5% -97.3% with relative standard deviations <4.9% (n = 6). The method has good reproducibility and is suitable for the detection of the above 16 kinds of synthetic colorants in various foods.