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本文使用Fura-2做为细胞内钙离子荧光指示剂,在大白鼠胰腺分离细胞观察缩胆囊素和乙酰胆碱对细胞内钙离子的影响及其相互作用.结果表明1nmol/L缩胆囊素和lmmol/L乙酰胆碱分别使细胞内钙离子浓度从基础水平的122±8nmol/L增高至360±32nmol/L和380±20nmol/L,半最大有效浓度分别为0.45nmol/L及54μmol/L。不同剂量的缩胆囊素和乙酰胆碱彼此不同程度抑制对方的作用,后加入的试剂提高细胞内钙离子浓度的幅度与先加入的试剂所引起的增加幅度成反比,加入各自相应的受体拮抗剂可使细胞恢复对另一激素(蛙皮素)刺激的反应。说明了缩胆囊素受体和胆碱能受体的相互调节影响细胞内钙离子的活动.加入受体拮抗剂后,细胞恢复反应过程中的时间动力学改变可能与受体阻滞后钙离子通道关闭的程度和时间有关.
In this article, Fura-2 was used as intracellular calcium ion fluorescent indicator. The effects of cholecystokinin and acetylcholine on intracellular calcium were observed in rat pancreas isolated cells. The results showed that 1nmol/L cholecystokinin and lmmol/ L-acetylcholine increased the intracellular calcium concentration from the basal level of 122±8 nmol/L to 360±32 nmol/L and 380±20 nmol/L, respectively, and the half maximal effective concentrations were 0.45 nmol/L and 54 μmol/L, respectively. Different doses of cholecystokinin and acetylcholine inhibit each other’s effects to different extents. The addition of reagents to increase the intracellular calcium ion concentration is inversely proportional to the increase caused by the previously added reagents. The addition of their respective receptor antagonists can The cells are restored to their response to another hormone (bombesin) stimulation. It shows that the mutual regulation of cholecystokinin receptor and cholinergic receptor affects the activity of intracellular calcium. After the addition of receptor antagonists, the temporal dynamics during cell recovery may be related to the receptor blocking calcium ion. The degree of closure of the channel is related to time.