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本室曾报道ＲＡ５３８转染亲本食管癌细胞系ＥＣ８７１２，可使后者发生终末分化和凋亡。为进一步研究这个ｃＤＮＡ的生物学作用，用ＲＡ５３８０．３ｋｂ的片段与ｎｅｏ基因构建了一个ｐＲＡ５３８－０．３－ｎｅｏ表达质粒，并转入三种不同的人癌细胞系即ＥＣ８７１２、ＨＬ６０和ＧＬＣ（一个来自人肺腺癌的细胞系）。经含Ｇ４１８培养液筛选后，进行了生长速度、３Ｈ－ＴｄＲ掺入率、细胞形态学、在半固体琼脂中克隆形成能力，以及裸鼠异种接种等检测。对获得的抗Ｇ４１８细胞群体均用上述０．３ｋｂ片段进行细胞原位杂交以证明其掺入和表达。同三个亲本癌细胞系比较这些细胞群体的生长和恶性表型均受到明显的抑制。
We have reported that RA538 transfects the parental esophageal cancer cell line EC8712, which can cause terminal differentiation and apoptosis. To further study the biological role of this cDNA, a pRA538-0.3-neo expression plasmid was constructed using the RA538 0.3 kb fragment and the neo gene, and transferred into three different human cancer cell lines, EC8712, HL60, and GLC ( A cell line derived from human lung adenocarcinoma). After screening with G418 culture medium, growth rate, 3H-TdR incorporation rate, cell morphology, colony formation ability in semi-solid agar, and heterologous inoculation of nude mice were examined. In situ hybridization of the above-mentioned 0.3 kb fragment against the obtained anti-G418 cell population was performed to prove its incorporation and expression. The growth and malignant phenotypes of these cell populations were significantly inhibited compared to the three parental cancer cell lines.