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本文使用PCR—RFLP方法分析了42例福尔马林固定、石蜡包埋胃癌组织中c-Ha-Yas基因第12位和61位密码子、N-ras基因第12位密码子、K-ras基因第12位和第13位密码子的点突变。发现14例(33.3％)有c-Ha-ras基因第12位密码子的点突变、2例(4.8％)有K-ras基因第12位密码子的点突变,且点突变与胃癌患者的预后有关。我们认为,ras基因点突变的检测可成为临床医生判断胃癌病人预后有价值的指标。
The PCR-RFLP method was used to analyze the codons 12 and 61 of c-Ha-Yas gene, the 12th codon of N-ras gene, and K-ras in 42 formalin-fixed, paraffin-embedded gastric cancer tissues. Point mutations at the 12th and 13th codons of the gene. A point mutation in codon 12 of the c-Ha-ras gene was found in 14 cases (33.3%) and a point mutation in codon 12 of K-ras gene in 2 cases (4.8%). The prognosis is related. We believe that the detection of ras point mutations can be a valuable indicator for clinicians to judge the prognosis of gastric cancer patients.