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A sensitive nicotine sensor based on a molecularly imprinted electropolymer of o-aminophenol is proposed and its configuration and performance are studied in detail.On the condition of weak acidity,the sensitive layer was prepared by electropolymerization of o-aminophenol on a gold electrode in the presence of the template(nicotine).The sensor exhibits good selectivity and sensitivity to nicotine.The determination limit is 2.0×10-7 mol/L and a linear relationship between the current and concentration is found in the range of 4.0x10-7～3.3 x10-5 mol/L.The sensor has also been applied to the analysis of nicotine in tobacco samples with recovery rates ranging from 99.0%to 102%.