介绍了用测量~3H标记的底物被分解的量反映肠匀浆和血浆中二胺氧化酶活性的方法。条件实验结果表明,血浆和肠匀浆分别保温1～5h和0.25～5h,分解的~3H标记底物的dpm随保温时间延长而增高,不同稀释浓度的肠匀浆分解~3H标记的底物,也随稀释倍数增加而降低。取样后保存时间对酶活性影响较大,取样后,1d内可测得满意的结果。 A method for measuring the activity of diamine oxidase in intestinal homogenate and plasma by measuring the amount of ~ 3H labeled substrate that is decomposed is described. The experimental results showed that plasma and intestinal homogenate incubated for 1 ~ 5h and 0.25 ~ 5h respectively, the dpm of decomposed ~ 3H labeled substrate increased with the prolongation of incubation time, and ~ 3H labeled substrate was decomposed by different concentrations of intestinal homogenate , Also decreased with increasing dilution factor. Sampling time after the preservation of the greater impact on enzyme activity, sampling, 1d can be measured within satisfactory results.