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目的维甲酸类化合物能预防头颈部癌前病变转变为癌，并能有效地降低头颈部第二原发癌的发生率。近来研究证实，维甲酸对头颈部鳞癌有诱导分化治疗作用，但维甲酸对头颈部腺癌诱导分化作用研究少见报道。方法本研究采用克隆形成法、ＭＴＴ检测法以及细胞周期测定技术，把全反式维甲酸（ａｌｔｒａｎｓｒｅｔｉｎｏｉｃａｃｉｄＡ－ＴＲＡ）对人涎腺腺样囊性癌细胞株（ＡｃｃＭ）的体外抗增殖作用和细胞增殖周期进行了观察。结果显示，ＡＴＲＡ对ＡｃｃＭ细胞有一定抗增殖作用，且该作用与浓度、时间呈依赖关系；ＡｃｃＭ细胞经ＡＴＲＡ作用７２小时后其Ｓ期比例明显下降，同时Ｇ０＋Ｇ１期比例上升。结论研究表明，５～２０μｍｏｌ／Ｌ浓度的ＡＴＲＡ对ＡｃｃＭ细胞产生抗增殖作用。
The purpose of retinoids can prevent head and neck precancerous lesions into cancer, and can effectively reduce the incidence of second primary head and neck cancer. Recent studies have confirmed that retinoic acid on the differentiation of head and neck squamous cell carcinoma have a therapeutic effect, but retinoic acid on the differentiation of head and neck adenocarcinoma differentiation rarely reported. Methods In this study, we used clonogenic assay, MTT assay and cell cycle assay to investigate the anti-proliferation and cell proliferation of human salivary adenoid cystic carcinoma cell line (AccM) induced by altransretinoicacid A-TRA in vitro. The cycle was observed. The results showed that ATRA had a certain anti-proliferation effect on AccM cells, and the effect was dependent on the concentration and time. After ATRA treatment for 72 hours, the proportion of S phase in AccM cells was significantly decreased while the proportion of G0 + G1 phase increased. Conclusion The results showed that 5 ~ 20μmol / L ATRA had antiproliferative effect on AccM cells.