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目的：研究人类乳头状瘤病毒（ＨＰＶ）与肺鳞癌发生的病因关系。方法：采用聚合酶链反应（ＰＣＲ）技术检测石蜡包埋肺鳞癌组织标本５０份，肿瘤组织旁鳞状化生上皮标本３０份，正常支气管粘膜３０份中的ＨＰＶＤＮＡ。结果：上述三种标本中，ＨＰＶＤＮＡ的检出率分别为２６％、３７％和１０％，其中以ＨＰＶ１６型所占比例最高。结论：结果表明ＨＰＶ感染与肺鳞癌的发生有一定的联系，鳞状上皮化生似可视为癌前病变，ＰＣＲ技术较体外杂交技术和ＤＮＡ探针有更高的敏感性
Objective: To study the etiology of human papillomavirus (HPV) and lung squamous cell carcinoma. METHODS: Polymerase chain reaction (PCR) was used to detect 50 paraffin-embedded lung squamous cell carcinoma specimens, 30 specimens of squamous metaplastic epithelium beside the tumor tissue, and HPV DNA in 30 specimens of normal bronchial mucosa. Results: Among the above three types of samples, the detection rates of HPV DNA were 26%, 37% and 10%, respectively, of which HPV16 accounted for the highest proportion. Conclusions: The results suggest that HPV infection is associated with the development of lung squamous cell carcinoma. Squamous metaplasia may be seen as a precancerous lesion. PCR technique has higher sensitivity than in vitro hybridization and DNA probes.