以往在半永久及永久性水体中释放奈氏索科线虫的实验中,线虫在蚊类幼虫中的寄生率很高,并曾有发现多种蚊种自然感染该种线虫的报道。本文作者在路易斯安那选择潮水与涨后形成的2类孳生场所,包括6处露天牧场和多草的地区以及11处林区内的孳生地,面积为200～600平方呎之间进行试验。将培养7～8周含有线虫成虫和虫卵的15克湿沙土,撒布在有草和其他有机物质,无水而潮湿的孳生场所。在每次潮水上涨后24小时或更长时间,采集蚊类幼虫,用显微 In the past, nematodes were released from mosquito larvae in semi-permanent and permanent water bodies with high parasitic rates. Mosquito nematodes were found naturally in mosquito species. The authors tested two types of breeding grounds in Louisiana, including open-air pastures and grassy areas, and breeding grounds in 11 forest areas, with an area of ​​between 200 and 600 square feet. Fifteen grams of wet sand, containing nematodes and eggs, will be cultivated for seven to eight weeks and sprinkled on grass and other organic material in dry and damp breeding grounds. Mosquito larvae were harvested 24 hours or more after each tide of the tide and were microscopically observed