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本文参照Rummage JA等人(1985)报道的方法,建立了检测巨噬细胞吞噬功能的微量比色法。用以观察生育、不育男性精浆中的免疫抑制物质对小鼠腹腔巨噬细胞(PEC-Mφ)吞噬功能的影响,结果是不育男性精浆作用过的PEC-Mφ吞噬SRBC百分率显著高于生育组(P<0.01)。并与抗补体法检测精浆免疫抑制物质溶血活性进行了比较,结果呈负相关关系(r=-0.89)。
In this paper, a micro-colorimetric assay for detecting the phagocytosis of macrophages was established by the method reported by Rummage JA et al. (1985). To observe the effects of immunosuppressive substances in fertile and infertile male seminal plasma on the phagocytosis of mouse peritoneal macrophages (PEC-Mφ), the result showed that the percentage of phagocytic SRBC of PEC-Mφ induced by infertile male seminal plasma was significantly higher In the fertility group (P <0.01). The anti-complement assay was used to detect the hemolytic activity of immunosuppressive substances in seminal plasma. The results showed a negative correlation (r = -0.89).