目的:探讨昆明山海棠片微生物限度检查方法的准确性和可靠性,并对该品种的微生物限度检查方法进行方法学验证。方法:采用菌液组、供试品对照组、试验组、稀释剂对照组进行3次独立平行试验,分别计算各试验菌每次试验的回收率。结果:各试验菌的回收率均高于70%,稀释剂对5种试验菌的回收率均高于70%。结论:采用常规法可以进行本品的微生物限度检查。 OBJECTIVE: To investigate the accuracy and reliability of the method for determining the limit of microbial population in Begonia tuberosum L., and to verify the method of microbial limit test of this species. Methods: The bacterial liquid group, for the test control group, test group, diluent control group three independent parallel tests were calculated for each test bacteria recovery of each test. Results: The recoveries of all tested bacteria were higher than 70%, and the recoveries of all five tested bacteria were higher than 70%. Conclusion: The conventional method can be used to check the limits of the microbial products.