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目的:探讨米非司酮(MIF)对人子宫颈癌Hela细胞的凋亡诱导作用。方法:体外培养人子宫颈癌Hela细胞,采用流式细胞术分析不同浓度MIF对Hela细胞的凋亡率及细胞周期分布的影响;采用Westernblot法检测MIF对Hela细胞中NF-κBp65蛋白表达的影响。结果:3个浓度的MIF均能使Hela细胞凋亡率明显增加,使S期、G2/M期细胞比例减少,G0/G1期细胞比例显著增加,且作用呈剂量依赖性;随着MIF浓度的增加,Hela细胞中NF-κBp65蛋白表达水平逐渐下降。结论:MIF可能通过NF-κB信号通路降调NF-κBp65蛋白表达,从而调节细胞周期、诱导Hela细胞凋亡。
AIM: To investigate the apoptosis-inducing effect of mifepristone (MIF) on human cervical carcinoma Hela cells. Methods: Human cervical carcinoma Hela cells were cultured in vitro. The effects of different concentrations of MIF on the apoptosis rate and cell cycle distribution of Hela cells were analyzed by flow cytometry. The effect of MIF on the expression of NF-κB p65 in Hela cells was detected by Western blot . Results: All the three concentrations of MIF could significantly increase the rate of apoptosis in Hela cells, and decrease the proportion of cells in S phase and G2 / M phase, and increase the proportion of cells in G0 / G1 phase in a dose-dependent manner. With MIF concentration , The expression of NF-κBp65 in Hela cells gradually decreased. Conclusion: MIF may down-regulate the expression of NF-κBp65 through NF-κB signaling pathway, thereby regulating cell cycle and inducing Hela cell apoptosis.