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利用聚丙烯酰胺凝胶电泳对两个玉米自交系作酯酶(EST)同工酶测定。结果表明:以单粒种子为样品进行酯酶同工酶分析,酶谱清晰,重现性好;通过比较它们的酶谱差异,计算各试材的酶谱相似系数,发现1和4的2种酶谱一致,2和3的2种酶谱一致应是同一样品;1与2的亲缘关系很近,应该是有一定亲缘关系的不同类型。通过对试材的植物学性状比较,所得结果与酶谱分析基本一致,并讨论了它们在制种上的应用以及认为酯酶同工酶作为鉴定玉米自交系亲缘关系及真实性的方法切实可行,具有快速、准确之优点。
Esterase (EST) isozymes were determined on two maize inbred lines by polyacrylamide gel electrophoresis. The results showed that the single enzyme seed samples were used for the analysis of esterase isozymes, the enzyme spectrum was clear and the reproducibility was good. By comparing their zymogram differences, the similarity coefficients of the enzymes in each sample were calculated and found that 1 and 4 of 2 The same zymogram, 2 and 3 of the two zymograms should be consistent with the same sample; 1 and 2 are closely related, it should be a certain kinship of different types. Through the comparison of the botanical characters of the tested materials, the obtained results are basically the same as those of the zymogram analysis, and their application in the seed production and the method of using the esterase isoenzyme as the identification of the genetic relationship and the authenticity of the maize inbred lines Feasible, with fast, accurate advantages.