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用长片段高保真度ＰＣＲ扩增系统（ｅｘｐａｎｄＴＭｈｉｇｈｆｉｄｅｌｉｔｙＰＣＲｓｙｓｔｅｍ）从鼻咽癌（ＮＰＣ）体外传代细胞株ＳＵＮＥ中扩增出ＥＢ病毒（ＥＢＶ）潜伏膜蛋白（ＬＭＰ１）全基因，经凝胶电泳分析和ＤＮＡ斑点杂交进行鉴定。扩增片段长度为３．４３ｋｂ，相当于Ｂ９５－８细胞ＥＢＶ基因序列位置为１７００３３～１６６６０８ｎｔ，包括ＬＭＰ１基因的上游启动子／增强子序列、ＬＭＰ１编码序列的３个外显子、两个内含子及下游ｐｏｌｙＡ信号序列。ＬＭＰ１全基因的扩增对于深入研究ＬＭＰ１基因的结构与功能以及ＬＭＰ１在ＮＰＣ发生发展中的作用机制具有十分重要的价值。
The EBV (LMP1) latent membrane protein (LMP1) gene was amplified from nasopharyngeal carcinoma (NPC) in vitro cell line SUNE by using expandTM high fidelity PCR system and analyzed by gel electrophoresis and DNA Dot blot identification. The amplified fragment length of 3.43kb, corresponding to B95-8 cells EBV gene sequence position of 170033 ~ 166608nt, including LMP1 gene upstream promoter / enhancer sequence, LMP1 coding sequence of three exons, two containing And downstream polyA signal sequence. The amplification of LMP1 gene is of great value to further study the structure and function of LMP1 gene and the mechanism of LMP1 in the development and progression of NPC.