随着培养时间的延长，日本血吸虫童虫培养细胞的超微结构发生一系列渐进性变化，主要表现在：核中异染色质含量逐渐增加，由０ｄ时的显著少于常染质转变为５４ｄ时的高于常染色质；线粒体逐渐变性，嵴由清晰变为模糊以至消失，基质电子密度降低，最终成为空泡；内质网由环状变为短管状、囊泡状，最后消失。日本血吸虫童虫培养细胞中线粒体和内质网超微结构的这些变化，可以用来判断培养条件的优劣；而从染色质的一系列变化推断，０～１８ｄ是诱导培养细胞发生增殖的最佳时期。 With the extension of culture time, the ultrastructure of Schistosoma japonicum Schistosoma japonicum cultured cells underwent a series of gradual changes, mainly manifested in: the nuclear heterochromatin content increased gradually from 0d significantly less than the change of the euchromatin to 54d When the mitochondria gradually degenerated, the cristae changed from clear to vague and even disappeared, the matrix electron density decreased and eventually became vacuoles. The endoplasmic reticulum changed from ring-shaped to short-tube and vesicular and finally disappeared. These changes of mitochondria and endoplasmic reticulum ultrastructure in cultured Schistosoma japonicum Schistosoma japonicum could be used to judge the quality of culture conditions. From the series of chromatin changes, it was concluded that 0-18 days was the most induced proliferation of cultured cells Good times.