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目的:分析肺腺癌组织中RNA结合基序蛋白38基因(RNA-binding motif protein 38,RBM38)及抑癌基因p53 m RNA和蛋白的表达情况,探讨其在肺腺癌发生发展中的意义。方法:取2012年10月至2015年6月新疆医科大学附属肿瘤医院收治的50例肺腺癌患者的肿瘤组织标本作为实验组,相对应的癌旁组织标本作为对照组。用RT-PCR法检测两组中RBM38及p53m RNA相对表达量,用Western blotting法检测两组中RBM38及p53蛋白的相对表达量。结果:实验组RBM38 m RNA及蛋白相对表达量(0.357±0.170、0.294±0.149)均高于对照组(0.271±0.128、0.206±0.099),实验组p53 m RNA及蛋白(0.457±0.208、0.671±0.200)相对表达量均高于对照组(0.308±0.167、0.332±0.071),差异均有统计学意义(均P<0.01)。RBM38表达与肺腺癌患者TNM分期、浸润深度有关(P<0.05),p53表达与患者TNM分期有关(P<0.05)。实验组RBM38与p53蛋白表达呈负相关(r=-0.626,P<0.01)。结论:肺腺癌组织RBM38、p53 m RNA及蛋白表达均高于癌旁组织,两者均与患者TNM分期等病理参数关系密切。随着RBM38蛋白表达的增加p53蛋白随之减少,RBM38可能通过抑制p53的翻译从而促进肺癌的发生发展,RBM38可能成为肺腺癌分子靶向治疗的靶点。
OBJECTIVE: To analyze the expression of RNA-binding motif protein 38 (RBM38) and p53 tumor suppressor gene p53 mRNA and protein in lung adenocarcinoma and to explore its significance in the development of lung adenocarcinoma. Methods: Tumor tissue specimens of 50 patients with lung adenocarcinoma admitted to Cancer Hospital Affiliated to Xinjiang Medical University from October 2012 to June 2015 were selected as the experimental group, and the corresponding paracancerous tissue specimens were taken as the control group. The relative expression of RBM38 and p53 mRNA in the two groups was detected by RT-PCR. The relative expression of RBM38 and p53 protein in the two groups was detected by Western blotting. Results: The relative expression of RBM38 mRNA and protein in the experimental group (0.357 ± 0.170,0.294 ± 0.149) was significantly higher than that in the control group (0.271 ± 0.128,0.206 ± 0.099). The levels of p53 mRNA and protein in the experimental group were (0.457 ± 0.208,0.671 ± 0.200) were higher than the control group (0.308 ± 0.167,0.332 ± 0.071), the differences were statistically significant (all P <0.01). The expression of RBM38 was correlated with TNM stage and depth of invasion (P <0.05) in patients with lung adenocarcinoma. The expression of p53 was correlated with TNM stage (P <0.05). There was a negative correlation between RBM38 expression and p53 protein expression in the experimental group (r = -0.626, P <0.01). CONCLUSION: The expressions of RBM38, p53 m RNA and protein in lung adenocarcinoma tissues are higher than that in paracancerous tissues, both of which are closely related to pathological parameters such as TNM stage. As the expression of RBM38 protein increases, the expression of p53 decreases. RBM38 may promote the development of lung cancer by inhibiting the translation of p53. RBM38 may be the target of molecular targeted therapy of lung adenocarcinoma.