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采用光镊拉曼光谱(LTRS)方法收集一例重型α地中海贫血患者单个红细胞的拉曼光谱,分析同一群体内不同细胞间的光谱差异,从中了解地中海贫血红细胞的病理过程。结果发现,重型α地中海贫血患者红细胞的拉曼光谱信号显著低于正常对照,并检测到一定比例的有核红细胞;正常对照的红细胞拉曼光谱均一,而重型α地中海贫血患者的细胞形态和拉曼光谱均显示出多样性;中等大小、形态接近正常的一类红细胞,其细胞间光谱差异最大;单细胞拉曼光谱可观察到部分外表形态正常的红细胞,其血红蛋白可能发生了血红素凝集和蛋白质变性。
Raman spectra of single red blood cells in a patient with severe α-thalassemia were collected by optical tweezers Raman spectroscopy (LTRS), and the differences of spectra among different cells in the same group were analyzed to understand the pathological process of thalassemia erythrocytes. The results showed that Raman spectra of erythrocytes in patients with severe α-thalassemia were significantly lower than those in normal controls, and a certain percentage of nucleated erythrocytes were detected. Raman spectra of erythrocytes in normal controls were homogeneous, whereas those in patients with severe α-thalassemia Man spectrum showed diversity; medium size, normal morphology of a class of red blood cells, the largest difference between the cell spectra; single cell Raman spectroscopy can be observed in part appearance of normal red blood cells, hemoglobin hemoglobin aggregation and Protein denaturation.