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目的:研究神香草与大苞荆芥乙醇提取物不同极性部位的体外抗氧化活性。方法:两种药材乙醇提取物依次用石油醚、二氯甲烷、乙酸乙酯和正丁醇萃取。采用DPPH·自由基法、邻苯三酚自氧化法、铁离子还原法、羟基自由基法等方法,以维生素C(Vc)为参照,分别研究两种药材不同萃取物的抗氧化活性。并计算各组分的IC50。结果:神香草和大苞荆芥乙醇提取物的不同萃取部分具有不同程度的清除自由基能力和还原能力,其中用DPPH法测得神香草各萃取部分半数抑制率(IC50)分别为:HCA(0.201 7),HCP(0.930 6),HCC(0.918 5),HCE(0.028 2),HCB(0.097 4),HCW(0.255 0)g·L-1;大苞荆芥各萃取部分IC50分别为:HbA(0.147 6),NbP(0.124 9),Nb(0.331 1),NbE(0.047 2),NbB(0.089 4),NbW(0.190 8)g·L-1。结论:神香草与大苞荆芥均具有一定抗氧化活性,为进一步分离抗氧化的成分提供了实验依据。
OBJECTIVE: To study the antioxidant activity in vitro of different polar fractions of ethanol extract of Schisandra chongqing and Bracts. Methods: The two kinds of herbs ethanol extract were extracted with petroleum ether, dichloromethane, ethyl acetate and n-butanol. The antioxidant activities of different extracts of two herbs were studied by DPPH · free radical method, pyrogallol autoxidation method, iron ion reduction method, hydroxyl radical method and vitamin C (Vc) as reference. And calculate the IC50 of each component. Results: Different extracts of ethanol extract of Herba Vanuatu and Bractsia stellera had different levels of free radical scavenging ability and reducing ability. Among them, the half-value inhibition ratio (IC50) measured by DPPH method was: HCA ( (P <0.01), HCP (0.918 5), HCE (0.028 2), HCB (0.097 4) and HCW (0.255 0) g · L-1, respectively. HbA (0.147 6), NbP (0.124 9), Nb (0.331 1), NbE (0.047 2), NbB (0.089 4), NbW (0.190 8) g · L -1. CONCLUSION: Both Herba divaricata and Euphorbia pulcherrimus have certain antioxidant activity, which provides an experimental basis for further separation of antioxidant components.