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目的探讨恶性血液病患者外周血细胞DNA含量的变化在诊治中的意义。方法采用FCM检测111例患者外周血单个核细胞的DNA含量并进行分析比较。结果(1)恶性血液病组处于增殖期的S、G2/M期细胞DNA含量明显高于恶性肿瘤组及非恶性肿瘤组,而G0/G1期细胞的DNA含量相对低于后两组,P均<0.01。(2)初发组及复发组患者S、G2/M期细胞DNA含量及PI明显高于正常对照组和缓解组,P<0.05或<0.01。(3)不同病种间S期DNA含量及PICL>AL>NHL,但前两组间比较P>0.05,前两组与后一组比较P分别<0.01和<0.05。结论FCM检测外周血细胞DNA含量既能反映患者血细胞的增殖状况,也能随时进行动态监测,指导治疗。
Objective To investigate the significance of changes in DNA content in peripheral blood cells of patients with hematologic malignancies during diagnosis and treatment. Methods The DNA content of peripheral blood mononuclear cells in 111 patients was detected by FCM and analyzed. Results (1) The DNA content of S, G2/M phase cells in the proliferative phase of malignant hematologic disease group was significantly higher than that of malignant tumor group and non-malignant tumor group, while the DNA content of G0/G1 phase cells was relatively lower than that of the latter two groups. Both <0.01. (2) The DNA content and PI of S and G2/M phase cells in the primary and recurrent patients were significantly higher than those in the normal and remission groups, P<0.05 or <0.01. (3) DNA content in S phase and PICL>AL>NHL in different disease groups, but P>0.05 in the former two groups, P<0.01 and <0.05 in the former two groups and the latter group, respectively. Conclusion FCM detection of peripheral blood cell DNA content can reflect the proliferation of blood cells in patients, but also can be dynamically monitored at any time to guide treatment.