THE ENHANCED GREEN FLUORESCENT PROTEIN AS A MARKER FOR HUMAN TUMOR CELLS LABELLED BY RETROVIRAL TRAN

来源 :中国癌症研究：英文版 | 被引量 : 0次 | 上传用户：sjcameadow

【摘要】

：

Objective: To investigate the feasibility of marking the human tumor cells with enhanced green fluorescent protein (EGFP) in vitro. Methods: The retroviral vect

【作者】

：

傅建新王玮白霞卢大儒阮长耿陈子兴

【机构】

：

JiangsuInstituteofHematology,InstituteofGenetics

【出处】

：

中国癌症研究：英文版

【发表日期】

：

2002年2期

【关键词】

：

Green FLUORESCENT protein Gene transfer RETROVIRAL vector CULTURED tumor cell

【基金项目】

：

photographical artwork

下载到本地 , 更方便阅读

下载此文赞助VIP

声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架

论文部分内容阅读

Objective: To investigate the feasibility of marking the human tumor cells with enhanced green fluorescent protein (EGFP) in vitro. Methods: The retroviral vector LGSN encoding EGFP was constructed and three human tumor cell lines were infected with LGSN

其他文献

Fe^3＋掺杂钛酸铅陶瓷的制备及性能研究

采用溶胶凝胶（sol-gel）法制备Fe^3＋掺杂钛酸铅超细粉体，并烧结成陶瓷。讨论了影响铁掺杂PbTiO3凝胶的不同因素，确定了制备陶瓷的最佳工艺条件。利用XRD对钛酸铅粉体进行物相分析，采

期刊

钛酸铅陶瓷SOL-GELXRDSEM介电性能PbTiO3 ceramics sol-gel XRD SEM dielectric consta

硅粉在直流氩等离子体中的刻蚀提纯

介绍了一种用直流氩等离子体对硅粉刻蚀提纯的方法。实验结果表明硅粉纯度可由99．6％提高到99．95％。处理后的硅粉还可进行熔化-固化-粉碎再处理，因此这是一种技术上可行的太阳级硅

期刊

粉粒表面刻蚀刻蚀提纯太阳级硅直流等离子体particulate surface etching etching purification solar

THE PRELIMINARY RESULTS OF TREATMENT OFADVANCED AND RECURRENT MALIGNANT LYMPHOMA BY BEAC REGIMEN SUP

Objective: High dose chemotherapy supported by autologous hematopoietic stem cells transplantation (AHSCT) has developed dramaticly in recent years and become t

期刊

LymphomaAUTOLOGOUShematopoieticStemcellsTransplantationHighdosechemo

DISTRIBUTION OF VEGF mRNA IN BREAST CANCER WITH NONRADIOACTIVE IN SITU HYBRIDIZATION AT ELECTRON MIC

Object: To localize the mRNA coding for VEGF at Ultrastractural level in human breast cancer by using digoxigenin-labeled cDNA probes. Methods: Nonradio- active

期刊

NonradioactiveinSITUhybridizationELECTRONmicroscopyVEGFmRNABREASTcan

La1＋xMg2-xNi9（x=0，0．5，1．0，1．5）合金的储氢性能及电化学性能研究

利用高频熔炼方法制备了La1＋xMg2-xNi9（x=0，0．5，1．0，1．5）系列合金，并对其进行了XRD分析和储氢容量及电化学性能测定。结果表明：随着La含量增大，合金中LaNi5和（La，Mg）Ni3相转变为LaNi3相，且Mg2

期刊

储氢合金合金结构电化学性能储氢容量hydrogen storage alloy alloy structure electrochemical pe

CHROMOSOME 3 MAY HARBOR MULTIPLE TUMOR SUPPRESSOR GENES ASSOCIATED WITH PRIMARY GLIOBLASTOMA MULTIFO

Objective: To investigate whether deletion of chromosome 3 is involved in the carcinogenesis of primary glioblastoma multiforme (GBM) and to localize the possib

期刊

LossofheterozygosityGlioblastomaTumorSUPPRESSORgeneCHROMOSOME3Loss o

NEW DRUG TARGETING TREATMENT - GLIVEC

This review evaluates the role of Glivec in the treatment of chronic myelogenous leukemia and other malignant tumors. Preclinical and clinical evidence showed t

期刊

药物靶向疗法恶性肿瘤PDGFR-Β病因学治疗学Protein tyrosine kinase Glivec ST1571 Targeting t

ISOLATION OF ENDOTOXIN-SPECIFIC ANTIBODIES BY SELECTION OF AN SINGLE CHAIN PHAGE ANTIBODY LIBRARY

Objective: To isolate murine anti endotoxin single chain phage antibody from a constructed library. Methods: Total RNA was firstly extracted from murine splenic

期刊

EndotoxinsPHAGEANTIBODYlibraryE.COLIEndotoxinsPhage antibody library E.

SCREENING OF DRUG RESISTANCE-RELATED GENES FROM HUMAN OVARIAN CANCER CELL LINE OC3/ADR BY DD-PCR

Objective: To screen novel genes related to adriamycin (Adr) resistance from human ovarian cancer resistance cell line OC3/Adr. Methods: Multidrug resistant ova

期刊

卵巢的瘤ADRIAMYCIN药抵抗mRNA微分显示Ovarian neoplasmsAdriamycinDrug-resistancemRNA

EXPRESSION OF P120ctn IN NON-SMALL-CELL LUNG CANCER: A CLINICOPATHOLOGICAL STUDY

Objective: To investigate the expression of p120ctn in non-small-cell lungcancer (NSCLC) and its relationship with clinicopathological factors and prognosis. Me

期刊

p120ctnNon-small-cellLUNGcancerImmunohistochemistryPROGNOSISp120ctnNon

THE ENHANCED GREEN FLUORESCENT PROTEIN AS A MARKER FOR HUMAN TUMOR CELLS LABELLED BY RETROVIRAL TRAN

与本文相关的学术论文