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目的 观察小鼠粒 巨噬细胞集落刺激因子 (mGM CSF)基因修饰的瘤苗体内免疫小鼠后的抗肿瘤效应以及对抗原提呈细胞数量和功能的影响。方法 应用腺病毒载体介导mGM CSF基因修饰FBL 3小鼠红白血病细胞 ,灭活后用作瘤苗腹腔免疫小鼠 ,检测免疫后体内诱导的细胞毒性T淋巴细胞 (CTL)活性 ,观察小鼠存活期 ;计数免疫后腹腔中抗原提呈细胞 (APCs)的数量 ,流式细胞仪分析腹腔贴壁细胞中 33D1+树突状细胞 (DCs)的含量及检测其体外刺激同种异体混合淋巴细胞反应的能力。结果 mGM CSF基因修饰瘤苗腹腔免疫的小鼠体内诱导出较高水平的CTL活性 ,并可全部抵抗亲本肿瘤细胞的再攻击 ;进一步分析表明瘤苗免疫后增加腹腔中APCs的数量及腹腔贴壁细胞中 33D1+DCs的含量 ,腹腔贴壁细胞体外刺激同种异体混合淋巴细胞反应的能力增强。结论 腺病毒载体介导mGM CSF基因修饰的瘤苗体内具有较强的抗肿瘤效应 ,此特异性抗肿瘤免疫功能的增强可能与瘤苗旁分泌mGM CSF ,促进瘤苗局部APCs的浸润及对肿瘤抗原的提呈有关
Objective To observe the anti-tumor effect of mice immunized with macrophage colony-stimulating factor (mGM CSF) gene in mice and the effect on the number and function of antigen-presenting cells. METHODS: Adenovirus vector-mediated mGM CSF gene was used to modify FBL 3 mouse erythroleukemia cells. After inactivation, mice were used as intraperitoneal immunized mice to measure the cytotoxic T lymphocyte (CTL) activity induced in mice after immunization. Survival; number of antigen-presenting cells (APCs) in the peritoneal cavity after enumeration of immunizations, flow cytometric analysis of 33D1+ dendritic cells (DCs) in peritoneal adherent cells and detection of their in vitro stimulation of allogeneic mixed lymphocyte responses Ability. RESULTS: MGM CSF gene-modified tumor vaccines induced higher levels of CTL activity in mice immunized intraperitoneally, and all of them could resist the re-attack of parental tumor cells. Further analysis showed that the number of APCs in the abdominal cavity and the abdominal adherence were increased after the vaccine immunization. The content of 33D1+DCs in the cells, and the ability of the abdominal adherent cells to stimulate the allogeneic mixed lymphocyte reaction in vitro. Conclusion Adenoviral vector-mediated mGM CSF gene modified tumor vaccines have strong anti-tumor effect. The enhanced anti-tumor immune function may be related to paracrine mGM CSF of tumor vaccines, promote the infiltration of local APCs in tumor vaccines and tumors. Related to the submission of antigens