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作者用多聚酶链反应(PCR)技术测定了19例急性淋巴细胞白血病(ALL)和15例慢性淋巴细胞白血病(CLL)患者在确诊时的外周血和骨髓的DNA 标本,还检测了5例正常人外周血的DNA 标本。白血病患者标本中均含有10%以上的白血病细胞。用V670/OL-4,VH26/OL-4两对引物来扩增免疫球蛋白重链基因的CDR■(Complementarity determining re-gion 3)区。依FAB 分型19例ALL 中L_1型3例,L_2型15例,L_3型1例。其免疫学表型为普通型-ALL(C-ALL)15例,T-ALL2例,B-ALL 和无标记ALL(N-ALL)各1例,15例CLL 均为B-免疫表型。C-ALL、B-ALL、N-ALL 共
The authors used polymerase chain reaction (PCR) technology to determine the DNA samples of peripheral blood and bone marrow in 19 cases of acute lymphoblastic leukemia (ALL) and 15 cases of chronic lymphocytic leukemia (CLL) at the time of diagnosis. Five normal people were also tested. Peripheral blood DNA specimens. Leukemia patients have more than 10% of leukemia cells in their specimens. Pairs of primers V670/OL-4, VH26/OL-4 were used to amplify the CDR (Complementarity determining re-gion 3) region of the immunoglobulin heavy chain gene. According to the FAB classification, 19 cases of ALL had L1 type 3 cases, L2 type 15 cases, and L3 type 1 case. The immunophenotypes were common-ALL (C-ALL) in 15 cases, T-ALL in 2 cases, B-ALL and unlabeled ALL (N-ALL) in 1 case, and 15 cases of CLL were all B-immune phenotypes. Total of C-ALL, B-ALL, N-ALL