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目的:研究金匮肾气丸对异丙肾上腺素致大鼠心室重构相关神经体液因子和形态学的改变。方法:异丙肾上腺素连续3 d以20,10,5 mg.kg-1递减皮下注射,3 mg.kg-1维持7 d制备大鼠心室重构模型,造模成功后分别用依那普利(2 mg.kg-1),金匮肾气丸(7.5 g.kg-1)ig给药5周及15周,从血浆中神经内分泌因子和形态学进行疗效比较研究。结果:血浆中神经内分泌因子的检测:5,15周时模型组血浆中心房钠尿肽(ANP)含量均高于正常组;5周时与模型组比较,金匮肾气丸组的ANP含量均较低(P<0.05);15周时,金匮肾气丸组的ANP、脑尿钠肽(BNP)含量均降低(P<0.05)。5周模型组的血清去甲肾上腺素(NE)浓度高于正常组,但无显著性差异,15周模型组的NE显著高于正常组(P<0.05);5,15周时,依那普利组和金匮肾气丸组的NE与模型组比较均无显著性差异。5,15周,模型组的血清血管紧张素Ⅱ(AngⅡ)浓度与正常组比较均无显著性差异。心肌病理形态学观测结果:5周时,模型组有较多成纤维细胞和新生毛细血管,肉芽组织较明显,15周时,模型组呈现广泛纤维化,形成疤痕组织;5,15周时,金匮肾气丸组的心肌细胞均有一定程度的病理变化,但较模型组而言均有改善。结论:金匮肾气丸能够改善异丙肾上腺素致大鼠心室重构过度激活的神经体液水平,但这种改善作用在晚期时较为明显。
OBJECTIVE: To study the effect of Jinkuishenqi Pill on the changes of ventricular remodeling-related neurohumoral fluid and morphology in rats induced by isoproterenol. METHODS: Isoproterenol was injected subcutaneously in the dose of 20, 10, 5 mg.kg-1 for 3 d continuously and the rat model of ventricular remodeling was established after 3 mg.kg-1 administration for 7 d. (2 mg.kg-1) and Jingui Shenqi Pill (7.5 g.kg-1) for 5 weeks and 15 weeks respectively. The neuroendocrine factors and morphological characteristics were compared between the two groups. Results: The neuroendocrine factors in plasma were detected at 5 and 15 weeks. The levels of plasma atrial natriuretic peptide (ANP) in the model group were higher than those in the normal group at 5 and 15 weeks. Compared with the model group, the ANP content (P <0.05). At 15 weeks, the content of ANP and BNP in Jinkuishenqi Pill decreased (P <0.05). The concentration of NE in the 5-week model group was higher than that in the normal group, but there was no significant difference. The NE in 15-week model group was significantly higher than that in the normal group (P <0.05). At 5 and 15 weeks, There was no significant difference between NE and model group in Puli group and Jinkuishenqi group. At 5 and 15 weeks, there was no significant difference between the model group and the normal group in serum Ang Ⅱ concentration. At the 5th week, the model group had more fibroblasts and newborn capillaries, and the granulation tissue was more obvious. At 15 weeks, the model group showed extensive fibrosis and formed scar tissue. At 5 and 15 weeks, Jinkuishenqi group of myocardial cells have a certain degree of pathological changes, but compared with the model group, both improved. Conclusion: Jinkuishenqi Pill can ameliorate the level of neurohumoral fluid that is over-activated by isoproterenol-induced ventricular remodeling in rats, but this improvement is more obvious in the late stage.