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根据纳米微粒在液相中的分散受表面润湿、粒子间作用能、双电层静电作用能、空间阻力作用能等因素影响,采用十二烷基苯磺酸钠、吐温80、硅酸钠复配,通过高速剪切与超声作用得到纳米银在水相中最佳的分散条件:硅酸钠、十二烷基苯磺酸钠、吐温80浓度(质量分数)分别为0.5%,0.5%,2%,超声时间为1.5h。透射电镜观测表明,纳米银在水相中分散稳定均匀。用琼脂稀释法测定最小抑菌浓度,纳米银分散液对大肠杆菌8099和金黄色葡萄球菌ATCC6538的最小抑菌浓度都为50μg/g。
According to the dispersion of nanoparticles in the liquid phase by the wetting of the surface, the interaction energy between particles, the electrostatic energy of the electric double layer, the space resistance effect and other factors, using sodium dodecyl benzene sulfonate, Tween 80, silicic acid Sodium complex, the best dispersion conditions of nano-silver in aqueous phase were got through high-speed shearing and ultrasonic interaction: sodium silicate, sodium dodecyl benzene sulfonate, Tween 80 concentration (mass fraction) were 0.5% 0.5%, 2%, ultrasonic time 1.5h. Transmission electron microscopy showed that nano-silver dispersed in the aqueous phase and stable. The minimum inhibitory concentration (MIC) was determined by agar dilution method. The minimum inhibitory concentration (MIC) of nano-silver dispersion on E. coli 8099 and Staphylococcus aureus ATCC6538 was 50μg / g.