论文部分内容阅读
目的采用高效液相色谱-二极管阵列检测方法,建立昆仑雪菊中绿原酸、芦丁、槲皮素和木犀草素的测定方法。方法色谱柱为安捷伦HC-C18柱(250 mm×4.6 mm,5μm);流动相为甲醇-0.01%磷酸水溶液,梯度洗脱;检测波长315nm;柱温30℃;体积流量1.0 m L/min。结果昆仑雪菊中绿原酸、芦丁、槲皮素和木犀草素分别在2.0~60μg/m L(r=0.999 8)、5.0~100μg/m L(r=0.999 9)、1.0~40μg/m L(r=0.999 9)和0.5~40μg/m L(r=0.999 9)呈良好的线性关系,平均回收率分别为102.4%(RSD为1.05%)、98.6%(RSD为1.28%)、103.6%(RSD为0.95%)、101.2%(RSD为1.12%)。结论该方法操作简便、易行、稳定,具有良好的灵敏性、重现性和回收率,可用于昆仑雪菊药材的质量控制。
OBJECTIVE To establish a method for the determination of chlorogenic acid, rutin, quercetin and luteolin in Kunlun Xueju by high performance liquid chromatography-diode array detection. Methods The column was Agilent HC-C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of methanol-0.01% phosphoric acid solution with gradient elution. The detection wavelength was set at 315 nm. The column temperature was 30 ℃ and the volume flow rate was 1.0 mL / min. Results The contents of chlorogenic acid, rutin, quercetin and luteolin in Kunlun Xueju were 2.0 ~ 60μg / m L (r = 0.999 8), 5.0 ~ 100μg / m L The average recoveries were 102.4% (RSD 1.05%) and 98.6% (RSD 1.28%), respectively. , 103.6% (RSD 0.95%), 101.2% (RSD 1.12%). Conclusion The method is simple, easy to operate, stable, has good sensitivity, reproducibility and recovery rate, which can be used for the quality control of Kunlun snowdrop medicinal materials.