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研究目的:生育酚是菜籽重要的品质指标,氮肥是影响作物生物学与籽粒产量最常用的肥料。这项研究旨在搞清楚氮肥种类与施量对油菜种子生育酚含量与组分的确切影响,以及这种影响在基因型之间的差异。创新要点:这篇论文研究了不同的氮肥种类(硝态氮与铵态氮)与低、中、高施用量对种子生育酚总量与组分的影响,并分析了其中的原因,为通过合理的氮肥施用方案配置,以达到最理想的菜籽生育酚含量或组分提供依据。研究方法:采用盆钵实验控制氮肥施量与流失的精准方法,五种基因型、二种氮肥种类、三档施量水平,三重复控制误差;尝试用气相色谱法检测菜籽生育酚含量的新方法。重要结论:尿素比硝酸氨更有利于菜籽总生育酚、阿尔法生育酚及伽马生育酚的有效形成;提高氮肥施量对于菜籽形成高含量的总生育酚与伽马生育酚非常有效,但对提高菜籽阿尔法生育酚含量的效果却不太明显。
Research purposes: Tocopherol is an important quality index of rapeseed, nitrogen fertilizer is the most commonly used fertilizer that affects crop biology and grain yield. The purpose of this study was to determine the exact impact of nitrogenous fertilizer application and dosage on the tocopherol content and composition of rapeseed seeds and the differences in genotypes of this effect. Innovative points: This paper studied the different types of nitrogen fertilizer (nitrate and ammonium nitrogen) and low, medium and high application rates on the total amount of tocopherols and components of the impact, and analyze the reasons for the adoption of Reasonable application of nitrogen fertilizer program configuration, in order to achieve the best rapeseed content or components provide the basis. Research methods: The precise method for controlling the amount and loss of nitrogen fertilizer, the five genotypes, two nitrogenous fertilizer types, the third gear application level and the triple repetition control error were studied by pot experiment. The content of tocopherol in rapeseed new method. Important Conclusions: Urea is more beneficial to the efficient formation of total tocopherols, alpha-tocopherols and gamma-tocopherols than ammonium nitrate. Increasing the amount of nitrogen fertilizers is very effective in forming high levels of total tocopherols and gamma-tocopherols in rapeseed, However, the effect of increasing rapeseed alpha-tocopherol content is less obvious.