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为研究槟榔碱 (Arecoline,ARE)与杀螺药物合用对钉螺足跖肌和神经节细胞超微结构的影响 ,探索其灭螺增效的作用机制。用加入和未加入 ARE的灭螺药物分别浸泡钉螺 2 4h后 ,运用透射电镜观察 2 5 mg/ L ARE单用或与杀螺药合用对钉螺足跖肌及神经节细胞超微结构的影响。结果 2 5 m g/ L ARE、0 .6 2 5 mg/ L 五氯酚钠(NAPCP)、0 .0 6 2 5 mg/ L 氯硝柳胺 (NIC)单用时 ,用药组钉螺足跖肌及神经节细胞超微结构与正常钉螺基本相同。而 2 5 mg/ L ARE分别与 0 .6 2 5 m g/ L NAPCP、0 .0 6 2 5 mg/ L NIC合用时 ,钉螺足跖肌及神经节细胞均发生明显改变。足跖肌纤维的肌丝被破坏 ,细胞器消失 ,核膜残缺 ,染色质凝集成团 ;神经节细胞高度变性坏死 ,成为一团低电子密度与高电子密度均匀混合的物质。结果表明 ,ARE能增强灭螺药对钉螺足跖肌和神经节细胞的破坏作用
In order to study the effects of Arecoline (ARE) and molluscicides on the ultrastructure of plantar muscle and ganglion cells of Scylla sinica, the mechanism of action of Arecoline (ARE) The snails were immersed in snails for 24 h with and without ARE, and the effects of 25 mg / L ARE alone or combined with control molluscicides on the ultrastructure of plantar muscle and ganglion cells were studied by transmission electron microscopy. Results When 25 mg / L ARE, 0.655 mg / L sodium pentachlorophenate (NAPCP) and 0. 0625 mg / L niclosamide (NIC) alone, The ganglion cells have the same ultrastructure as normal snails. When 25 mg / L ARE was combined with 0.6225 mg / L NAPCP and 0. 0625 mg / L NIC respectively, the plantar muscles and ganglion cells of Spina bifida were significantly changed. The myofilament of the plantar muscle fiber is destroyed, the organelles disappear, the nuclear membrane is incomplete, and the chromatin is agglutinated. The ganglion cells are highly degenerated and necrotic and become a mass of substances with low electron density and high electron density uniformly mixed. The results showed that ARE could enhance the destructive effect of molluscides on plantar muscle and ganglion cells