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目的:观察延胡索碱药物预处理对缺血再灌注心肌Ca2+-ATPase及Na+-K+-ATPase活性的影响。方法:将清洁级成年SD大鼠30只随机分为6组(延胡索碱高、中、低剂量预处理组,经典缺血预处理组,注射用水组和假手术组),结扎左冠状动脉前降支近段30min后再灌注60min,之后断头放血取心脏匀浆并测定心肌组织Na+-K+-ATP酶、Ca2+-ATP酶活性。结果:(1)Na+-K+-ATP酶活性变化:与假手术组比较,心肌缺血再灌注损伤后,心肌细胞膜Na+-K+-ATP酶活性显著下降(P<0.01)。与模型组比较,缺血预处理组和延胡索碱中、高剂量组的心肌细胞膜Na+-K+-ATP酶活力都明显升高(P<0.01);与缺血预处理组比较,延胡索碱中、高剂量组心肌细胞膜Na+-K+-ATP酶活性差异无显著性(P>0.05)。(2)Ca2+-ATP酶活性变化:与假手术组比较,心肌缺血再灌注损伤后,心肌细胞膜Ca2+-ATP酶活性显著下降(P<0.01)。与模型组比较,缺血预处理组和延胡索碱高剂量组的心肌细胞膜Ca2+-ATP酶活性都明显升高(P<0.05);与缺血预处理组比较,延胡索碱高剂量组心肌细胞膜Ca2+-ATP酶活性差异无显著性(P>0.05)。结论:延胡索碱药物预处理可提高心肌细胞Na+-K+-ATPase和Ca2+-ATPase活性,从而促进Na+-Ca2+交换,减轻细胞内Ca2+超载,保护缺血再灌注引起的心肌损伤。
Objective: To observe the effects of dietynic alkali pretreatment on the activity of Ca2 + -ATPase and Na + -K + -ATPase in ischemia-reperfusion myocardium. Methods: Thirty SD adult SD rats were randomly divided into 6 groups (high, medium and low dose pretreatment group, classic ischemic preconditioning group, water for injection group and sham operation group), ligation of left anterior coronary artery After 30 minutes of reperfusion, the rats were reperfused for 60 minutes after reperfusion. Then the hearts were homogenized by decapitation and the activities of Na + -K + -ATPase and Ca2 + -ATPase were determined. Results: (1) Na + -K + -ATPase activity changes: Na + -K + -ATPase activity was significantly decreased in myocardial cell membrane after myocardial ischemia-reperfusion injury compared with sham operation group (P <0.01). Compared with model group, the activity of Na + -K + -ATPase in ischemic preconditioning group and the fumaric alkali medium and high dose groups were significantly increased (P <0.01). Compared with ischemic preconditioning group, There was no significant difference in Na + -K + -ATPase activity in high-dose group (P> 0.05). (2) Ca2 + -ATPase activity changes: Compared with the sham operation group, myocardial cell membrane Ca2 + -ATPase activity decreased significantly after myocardial ischemia-reperfusion injury (P <0.01). Compared with model group, the activity of Ca2 + -ATPase in ischemic preconditioning group and high-dose fumarate group were significantly increased (P <0.05). Compared with ischemic preconditioning group, Ca2 + -ATP enzyme activity difference was not significant (P> 0.05). CONCLUSION: The fumaric alkali pretreatment can increase the activity of Na + -K + -ATPase and Ca2 + -ATPase in cardiomyocytes, promote the exchange of Na + -Ca2 +, decrease the intracellular Ca2 + overload and protect the myocardium from ischemia-reperfusion injury.