论文部分内容阅读
目的检测建鲤和黑龙江野鲤杂交与各自交F1代的肌肉营养成分。方法2008年8月25日～10月8日对三个群体进行了为期45d的网箱养殖之后,按GB/T5009-2003方法分别测定水分、粗蛋白、粗脂肪和粗灰分,用液相和气相色谱仪分别测定氨基酸和脂肪酸含量,用原子吸收光谱仪测定无机元素,分析比较三个群体的肌肉营养成分。结果杂交F1代的水分含量低于两自交群体,粗蛋白含量高于两自交群体,粗脂肪含量显著低于建鲤自交F1代而高于黑龙江野鲤自交F1代;氨基酸总量,杂交F1代较建鲤自交F1代有不同程度提升;杂交F1代的WEAA/WTAA和WEAA/WNEAA符合FAO/WHO标准,后者比两自交群体更为理想,其必需氨基酸指数EAAI(98.46),明显高于建鲤自交F1代(81.71);脂肪酸的含量,杂交F1代除在饱和脂肪酸的总量低于两自交群体外,单、多不饱和脂肪酸和必需脂肪酸总量均高于两自交群体;宏量和微量元素总量,杂交F1代均高于两自交群体。结论杂交F1代不仅在蛋白质营养价值上优于两自交群体,而且在脂肪酸和营养元素的含量上更为丰富。
Objective To detect the muscle nutrients of F1 hybrids between Jian carp and Heilongjiang wild carp and their crossbreds. Methods After three-day cage culture of three groups from August 25 to October 8, 2008, water, crude protein, crude fat and crude ash were determined according to GB / T5009-2003, Gas chromatographs were used to determine the content of amino acids and fatty acids respectively. The inorganic elements were determined by atomic absorption spectrometry. The nutritional compositions of the three groups were analyzed and compared. Results The F1 hybrids had lower water content than those of two inbreds, and the content of crude protein was higher than those of two inbreds. The crude fat content was significantly lower than that of F1 self-cross of Jian carp and F1 self-F1 of Heilongjiang wild carp. , F1 generation of hybrid F1 had more improvement than F1 generation of Jian carp. WEAA / WTAA and WEAA / WNEAA of F1 hybrid met the FAO / WHO standard, which was more ideal than that of two-hybrid population. The essential amino acid index EAAI ( 98.46), which was significantly higher than that of the Jianli self-F1 generation (81.71). The contents of fatty acids, the total number of mono-, polyunsaturated fatty acids and essential fatty acids in F1 hybrids except the total saturated fatty acids were lower than those in two-hybrid populations Higher than those of two inbreds; the total amount of macroscopic and trace elements in F1 hybrids were higher than those in two inbreds. Conclusion The hybrid F1 generation is not only superior to the two inbred populations in terms of protein nutritional value, but also enriched in the content of fatty acids and nutrients.