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目的研究大气细颗粒物对大鼠肺组织亚急性作用及其可能的作用机制。方法健康成年雄性Wistar大鼠35只随机分为空白对照组、生理盐水对照组、低剂量染尘组(1.5mg·kg-1·bw-1)、中剂量染尘组(7.5mg·kg-1·bw-1)、高剂量染尘组(37.5mg·kg-1·bw-1)。空白对照组不采取任何处理措施,生理盐水对照组用生理盐水进行气管滴注,将采集细颗粒物制成所需浓度的生理盐水混悬液对各剂量染尘组进行气管滴注。测定血清及灌洗液(BALF)中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、丙二醛(MDA)含量。结果空白对照组与生理盐水对照组各测定指标差异无统计学意义(P>0.05)。血清和灌洗液(BALF)中,各染尘组指标ACP、AKP、LDH、MDA与生理盐水对照组相比差异均有统计学意义(P<0.05),且存在剂量依赖关系。结论环境细颗粒物亚急性染尘可对大鼠肺组织造成一定程度的损伤作用。
Objective To study the subacute effect of fine particles of air on lung tissue of rats and its possible mechanism. Methods Thirty-five healthy adult male Wistar rats were randomly divided into control group, normal saline control group, low dose of dust group (1.5mg · kg-1 · bw-1), medium dose of dust group (7.5mg · kg- 1 · bw-1), high-dose group (37.5 mg · kg-1 · bw-1). The blank control group did not take any treatment measures, saline control group tracheal instillation with saline, the collection of fine particles made of the desired concentration of saline suspension for each dose of dust group intratracheal instillation. The levels of acid phosphatase (ACP), alkaline phosphatase (AKP), lactate dehydrogenase (LDH) and malondialdehyde (MDA) in serum and lavage fluid (BALF) Results There was no significant difference between the blank control group and the saline control group (P> 0.05). In serum and lavage fluid (BALF), the indexes of ACP, AKP, LDH and MDA in each dust group were significantly different from those in control group (P <0.05), and there was a dose-dependent relationship. Conclusion Sub-acute environmental dust particles can cause lung tissue damage in rats to a certain extent.