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目的观察难治性癫疒间患儿脑内病灶中脑红蛋白(NGB)的表达。方法选择2003年8~10月在北京天坛医院神经外科进行手术切除治疗的难治性癫疒间患儿7例,在对其临床资料分析的基础上,应用组织学染色观察手术切除标本(海马和颞叶)的病理改变;应用免疫组织化学方法,分别研究癫疒间患儿手术切除标本中海马和颞叶病灶NGB表达的变化情况,并进行半定量图像分析。结果7例标本中,均出现反应性胶质细胞的显著增生,具有难治性癫疒间共同的病理学特征。海马中NGB免疫反应阳性细胞明显少于颞叶病灶;且随发作年限增加而减少。结论难治性癫疒间患儿颞叶病灶NGB阳性细胞数多于海马,可能与发作时病灶处耗氧过度从而增加了对氧的需求有关。
Objective To observe the expression of NGB in intracerebral lesions in children with refractory epilepsy. Methods Seven children with intractable epilepsy who underwent surgical resection in Department of Neurosurgery, Beijing Tiantan Hospital from July to October in 2003 were selected. Based on the analysis of their clinical data, histological staining was used to observe the changes of the hippocampus And temporal lobe). Immunohistochemistry was used to study the changes of NGB expression in hippocampus and temporal lobe lesion in children with epilepsy and semi-quantitative image analysis. Results All the 7 specimens showed significant proliferation of reactive glial cells with common pathological features of intractable epilepsy. The number of NGB immunoreactive cells in the hippocampus was significantly less than that in the temporal lobe, and decreased with the increase of the attack time. Conclusion Compared with the hippocampus, the number of NGB positive cells in the temporal lobe lesions of children with refractory epilepsy may be related to the excessive consumption of oxygen in the lesion at the time of attack and the increase of oxygen demand.