本文介绍一种辅酶Ⅱ-黄递酶(NADPH-diaphorase)组织化学技术结合免疫细胞化学技术,用来研究脑内含有不同神经化学物质的神经元和纤维之间的相互关系。同一张组织切片先经过免疫细胞化学技术处理,显示出棕色的、某类神经递质(如:多巴胺)的成份。再经过NADPH-diaphorase组织化学处理,显示出一些染成兰色的神经元,这些神经元含有神经肽Y和生长抑制。这样,在同一张切片上即可分析两种或两种以上神经化学能成份之间的关系。 This article describes a coenzyme Ⅱ diaphorase (NADPH-diaphorase) histochemical technique combined with immunocytochemistry to study the relationship between neurons and fibers containing different neurochemicals in the brain. The same tissue section was first treated with immunocytochemistry to show the composition of a brown, certain neurotransmitter (eg, dopamine). Following NADPH-diaphorase histochemical processing, some dyed blue neurons were shown, which contain neuropeptide Y and growth inhibition. This way, you can analyze the relationship between two or more neurochemical components on the same slice.