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采用紫外-可见光谱、荧光光谱和傅里叶变换衰减全反射红外光谱等技术,研究在模拟人体生理Ph值条件下,丹参酚酸A(或丹参酚酸B)与胰岛素分子之间的结合作用,以及丹参酚酸A(或丹参酚酸B)对胰岛素二级结构的影响,并考察葡萄糖对它们的影响。实验结果表明,丹参酚酸A和丹参酚酸B均能导致胰岛素内源性荧光静态猝灭;同步荧光和三维荧光谱图表明胰岛素与丹参酚酸A(或丹参酚酸B)结合后,构象没有发生明显变化。红外光谱研究表明,胰岛素与丹参酚酸A(或丹参酚酸B)结合后二级结构发生了一些改变,β-转角和无规则卷曲的相对含量略有增加,α-螺旋和β-折叠没有发生明显改变。葡萄糖的加入会改变丹参酚酸A(或丹参酚酸B)与胰岛素的结合程度,并加剧胰岛素构象变化以及二级结构中α-螺旋相对含量改变,从而影响丹参酚酸A(或丹参酚酸B)-胰岛素体系中胰岛素的生物活性。
The binding of salvianolic acid A (or salvianolic acid B) to insulin was studied under the conditions of physiological Ph value by UV-Vis, fluorescence spectroscopy and Fourier transform attenuated total reflection infrared spectroscopy , And salvianolic acid A (or salvianolic acid B) on the secondary structure of insulin, and investigate the impact of glucose on them. The experimental results show that both salvianolic acid A and salvianolic acid B can lead to the static quenching of insulin endogenous fluorescence. Synchronous fluorescence and three-dimensional fluorescence spectroscopy show that after the binding of insulin with salvianolic acid A (or salvianolic acid B), the conformation No significant changes have taken place. The results of FTIR showed that the secondary structure of insulin changed slightly with the combination of salvianolic acid A (or salvianolic acid B), the relative content of β-turn and random coil slightly increased, but α-helix and β-sheet did not Significant changes have taken place. The addition of glucose will change the binding of salvianolic acid A (or salvianolic acid B) with insulin and aggravate the conformational changes of insulin and the relative content of α-helix in the secondary structure, thus affecting the metabolism of salvianolic acid A (or salvianolic acid B) - Insulin bioactivity in the insulin system.