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[目的]探讨锯叶棕提取物对RPMI8226细胞凋亡的影响以及对细胞凋亡相关蛋白表达的影响。[方法]①体外培养的RPMI8226细胞加入到不同浓度的锯叶棕提取物(0.5或1.0μL/mL)培养24h,利用流式细胞仪测定位于前G1时期的细胞百分比以检测细胞凋亡;②体外培养的RPMI8226细胞加入到锯叶棕提取物(1.0μL/mL)培养24h,应用Western Blot检测PARP、MCl-1蛋白表达。[结果]①锯叶棕提取物诱导RPMI8226细胞凋亡具有剂量依赖性(P<0.05);②RPMI8226细胞暴露于锯叶棕提取物(1.0μL/mL)培养24h,PARP蛋白水平表达明显增加,但不能调整Mcl-1。[结论]锯叶棕提取物诱导RPMI8226细胞凋亡。
[Objective] To investigate the effects of Saw Palmetto extract on the apoptosis of RPMI8226 cells and its effect on the expression of apoptosis-related proteins. [Methods] (1) RPMI8226 cells were cultured in different concentrations of Saw Palmetto (0.5 or 1.0 μL / mL) for 24 h. Flow cytometry was used to determine the percentage of cells in the pre G1 phase to detect apoptosis. RPMI8226 cells cultured in vitro were added with Saw Palmetto extract (1.0 μL / mL) for 24 h. The expression of PARP and MCl-1 protein was detected by Western Blot. [Results] ① Saw Palmetto extract induced apoptosis in RPMI8226 cells in a dose-dependent manner (P <0.05); ② RPMI8226 cells exposed to Saw Palmetto extract (1.0 μL / mL) for 24 h showed a significant increase in PARP protein expression Mcl-1 can not be adjusted. [Conclusion] Saw palmetten brown extract can induce apoptosis of RPMI8226 cells.