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本研究以NB4 ,HL 60细胞为研究对象 ,采用Wright′s染色法及荧光染色法、免疫荧光技术及RT PCR法 ,探讨雄黄对NB4 ,HL 60细胞的形态、PMLmRNA及蛋白的表达影响。结果发现 :①经雄黄处理后 ,各组均出现细胞凋亡的形态学上改变。②雄黄处理后NB4细胞内融合蛋白降解 ,PML蛋白聚积于POD结构 ,随后降解 ;在HL 60细胞中PML蛋白发生相似改变。③雄黄处理后各组细胞PMLmRNA表达均无显著影响。结论指出 ,在雄黄诱导下PML在蛋白水平参与诱导白血病细胞凋亡机制 ,雄黄能诱导NB4 ,HL 60细胞凋亡 ,抑制白血病细胞生长
In this study, NB4 and HL60 cells were studied using Wright’s staining and fluorescence staining, immunofluorescence, and RT PCR methods to investigate the effects of realgar on the morphology and PML mRNA and protein expression of NB4 and HL60 cells. The results showed that: 1 After treatment with realgar, there was a morphological change in apoptosis in each group. 2 The degradation of fusion protein in NB4 cells treated with realgar, PML protein accumulates in the POD structure, and then degrades; PML protein changes similarly in HL 60 cells. 3 The expression of PML mRNA had no significant effect on the cells after treatment with realgar. The conclusions indicate that PML is involved in the induction of apoptosis of leukemia cells at the protein level induced by realgar. Realgar can induce apoptosis of NB4 and HL60 cells and inhibit the growth of leukemia cells.