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目的:检测人贲门组织、贲门癌组织和食管组织、食管癌组织端粒酶RNA组分表达及端粒酶活性,探讨其在贲门癌和食管癌中的作用。方法:采用逆转录聚合酶链反应(RT-PCR)和端粒重复扩增法(TRAP)检测了人贲门组织、贲门癌组织和食管组织、食管癌组织端粒酶RNA组分及端粒酶活性。结果:在人贲门癌和食管癌组织中端粒酶RNA组分和端粒酶活性均呈阳性,人正常贲门和食管组织端粒酶活性呈阴性,端粒酶RNA组分呈阴性或低水平表达。结论:端粒酶在人贲门癌和食管癌的发生中可能起着重要作用。端粒酶活性的表达将成为人贲门癌和食管癌一项有用的诊断指标
OBJECTIVE: To detect the expression of telomerase RNA and telomerase activity in cardiac tissue, cardia cancer tissue, esophageal tissue and esophageal cancer tissue, and to explore its role in cardiac cancer and esophageal cancer. METHODS: Telomerase RNA components and telomerase were detected in human cardiac, cardiac, and esophageal and esophageal cancer tissues by reverse transcription polymerase chain reaction (RT-PCR) and telomere repeat amplification (TRAP). active. Results: The telomerase RNA and telomerase activity were positive in human cardiac and esophageal cancer tissues. The telomerase activity was negative in normal cardiac and esophageal tissues, and the telomerase RNA was negative or low. expression. Conclusion: Telomerase may play an important role in the occurrence of cardiac cancer and esophageal cancer. The expression of telomerase activity will be a useful diagnostic indicator for cardiac and esophageal cancer