采用高速逆流色谱技术分离提纯中药虎杖中的活性多酚成分白藜芦醇和白藜芦醇甙。确定了样品前处理方法 ,选择了逆流色谱流动相体系 ,经高效液相色谱检验纯度 ,1HNMR及质谱判定结构 ,证实所得样品为白藜芦醇和白藜芦醇甙 ,得率分别为2.18 %和1.07 %。 High-speed countercurrent chromatography was used to separate and purify the active polyphenols resveratrol and polydatin from the Chinese traditional medicine Polygonum cuspidatum. The sample pretreatment method was established, the mobile phase system of countercurrent chromatography was selected, and the purity, 1HNMR and MS were determined by high performance liquid chromatography. The obtained samples were confirmed to be resveratrol and polydatin with yields of 2.18% and 1.07%.