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目的建立一种定量测定艾片中l-龙脑、异龙脑和樟脑的方法。方法采用GC法测定艾片中l-龙脑、异龙脑和樟脑的量。分析柱为CP-WAX毛细管柱(30 m×0.32 mm,0.5μm);升温程序为80℃保持5 min,以10℃/min快速升温至220℃,保持5 min;进样分流比为1∶8;FID检测器温度为250℃;进样口温度为250℃;载气为高纯氮气,体积流量为3.0 mL/min,空气体积流量为400 mL/min,氢气体积流量为47 mL/min;进样量为1μL。结果樟脑、异龙脑和l-龙脑分别在0.10～8.08、0.10～7.98、0.10～8.16 mg/mL具有良好线性关系;平均回收率分别为97.52%、98.31%、98.16%,RSD分别为1.45%、1.18%、1.32%。结论本法操作简便、准确,精密度、分离度良好,分析时间短,为艾片的测定及其质量控制提供了参考。
Objective To establish a method for the quantitative determination of l-borneol, isoborneol and camphor in tablets. Methods The amount of l-borneol, isoborneol and camphor in the tablets of Alzheimer’s disease was determined by GC method. The analytical column was a CP-WAX capillary column (30 m × 0.32 mm, 0.5 μm). The temperature program was kept at 80 ℃ for 5 min and then rapidly heated up to 220 ℃ at 10 ℃ / min for 5 min. The injection split ratio was 1: 8; FID detector temperature is 250 ° C; inlet temperature is 250 ° C; carrier gas is high purity nitrogen, volume flow rate is 3.0 mL / min, air volume flow rate is 400 mL / min, hydrogen volume flow rate is 47 mL / min ; Injection volume of 1μL. Results There was a good linear relationship between camphor, isoborneol and l-borneol at 0.10 ~ 8.08, 0.10 ~ 7.98 and 0.10 ~ 8.16 mg / mL respectively. The average recoveries were 97.52%, 98.31% and 98.16%, respectively, and the RSDs were 1.45 %, 1.18%, 1.32%. Conclusion The method is simple, accurate, precise, good resolution and short analysis time, which provides a reference for the determination of AI and its quality control.