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利用分子标记辅助选择构建QPM近等基因系,以phi057为特异性引物,对90个回交群体各世代进行选择,构建出一批来自不同遗传背景的QPM近等基因系。结果表明,引物phi057在QPM与普通玉米自交系间表现多态性,能准确区分O2O2、O2o2和o2o2这3种基因型。因此,利用微卫星标记引物phi057在QPM与普通自交系杂交、回交世代中进行o2基因的选择是可行的。实验中的大部分材料在回交5～6代后与轮回亲本的农艺性状极为相似并稳定下来。经卡方检验,OO和Oo的分离比例为1∶1,符合孟德尔遗传分离比例。
The QPM near-isogenic lines were constructed with molecular marker-assisted selection. The phi057-specific primers were used to select the 90 backcross populations for each generation and a number of QPM near-isogenic lines from different genetic backgrounds were constructed. The results showed that the primer phi057 showed polymorphism between QPM and common maize inbred lines, which could accurately distinguish the three genotypes of O2O2, O2o2 and o2o2. Therefore, it is feasible to use the microsatellite marker phi057 to hybridize QPM with common inbred lines and backcross generation to select o2 gene. Most of the materials in the experiment were similar to the agronomic traits of the recurrent parents after backcrossing for 5-6 generations and stabilized. The chi-square test, OO and Oo separation ratio of 1: 1, in line with Mendelian genetic separation ratio.