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目的:研究异型南五味子丁素(HD)和戈米辛J(GJ)对豚鼠心肌L-型钙离子通道的作用。方法:全细胞膜片箝记录。结果:异型南五味子丁素1,10μmol/L及戈米辛J 10μmol/L可抑制L-型Ca~(2+)电流。HD和GJ对钙电流稳态激活都无影响,但它们可改变钙电流的稳态失活,提示两种药物作用于L-型钙通道的失活态。结论:HD和GJ对豚鼠心室肌细胞L-型钙离子通道有阻断作用。
Objective: To investigate the effects of dystrophic kadsura (HD) and gomisin J (GJ) on L-type calcium channel in guinea pig myocardium. Methods: Whole-cell patch-clamp recording. Results: L-type Ca2 + currents could be inhibited by 1, 10 and 10 micromol / L gomisin J 10 μmol / L. Both HD and GJ had no effect on steady-state calcium current activation, but they altered the steady-state inactivation of calcium current, suggesting that both drugs act on the inactivation state of the L-type calcium channel. Conclusion: HD and GJ can block the L-type calcium channel of guinea pig ventricular myocytes.