论文部分内容阅读
目的:建立消乳癖颗粒中甘露醇的HPLC-ELSD测定方法。方法:采用高效液相色谱法,蒸发光散射检测器。色谱柱:Carbomix-Ca柱(250 mm×4.6 mm,5μm);柱温:65℃;流动相:水,流速:0.6 ml·min~(-1);ELSD条件:漂移管温度:110℃,载气流速:3.0 L·min~(-1)。结果:甘露醇在0.554~9.964μg范围内线性关系良好,r=0.999 6,平均回收率为101.1%,RSD=1.67%(n=6)。结论:方法简便,结果准确,可用于测定消乳癣颗粒中甘露醇的含量。
Objective: To establish HPLC-ELSD method for the determination of mannitol in Xiao Ru Pi Ye. Methods: High performance liquid chromatography and evaporative light scattering detector were used. Column: Carbomix-Ca column (250 mm × 4.6 mm, 5 μm); column temperature: 65 ° C .; mobile phase: water; flow rate: 0.6 ml · min -1 ELSD conditions: drift tube temperature: Carrier gas flow rate: 3.0 L · min -1. Results: The linearity of mannitol in the range of 0.554-9.964μg was good, r = 0.999 6, the average recovery was 101.1%, RSD = 1.67% (n = 6). Conclusion: The method is simple and accurate. It can be used to determine the content of mannitol in ache ringworm granules.