论文部分内容阅读
目的:建立一种HPLC方法同时测定参麦地黄丸中丹皮酚、芍药苷和马钱苷的含量。方法:采用HPLC法,色谱柱为Agilent Zorbax SB-C18(4.6mm×250mm,5μm)柱,流动相:以乙腈-水为流动相,进行梯度洗脱;流速:1.0mL.min-1;柱温:30℃;检测波长:236nm(0～8min)→230nm(8～12min)→274nm(12～30min)。结果:丹皮酚、芍药苷和马钱苷线性范围分别为8.17～163.36μg·mL-1(r=0.9999),2.37～48.54μg·mL-1(r=0.9997)和2.71～31.82μg·mL-1(r=0.9998),平均加样回收率分别为98.62%(RSD=1.78%),97.58%(RSD=0.91%)和99.00%(RSD=2.56%)。结论:新建方法简便、准确、易行,可用于控制制剂质量。
Objective: To establish a HPLC method for simultaneous determination of paeonol, paeoniflorin and loganin in Shenmai Dihuang Pills. Methods: The HPLC method was used. The chromatographic column was Agilent Zorbax SB-C18 (4.6mm×250mm, 5μm) column. The mobile phase was acetonitrile-water as the mobile phase. Gradient elution was used. The flow rate was 1.0mL.min-1. Temperature: 30°C; Detection wavelength: 236 nm (0 to 8 min)→230 nm (8 to 12 min)→274 nm (12 to 30 min). RESULTS: The linear ranges of paeonol, paeoniflorin and logicoside were 8.17 to 163.36 μg·mL-1 (r=0.9999), 2.37 to 48.54 μg·mL-1 (r=0.9997) and 2.71 to 31.82 μg·mL, respectively. -1 (r = 0.9998), the mean sample recovery was 98.62% (RSD = 1.78%), 97.58% (RSD = 0.91%) and 99.00% (RSD = 2.56%), respectively. Conclusion: The new method is simple, accurate, and easy to use and can be used to control the quality of preparations.