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目的探测WHV/myc转基因小鼠肝癌发生过程中有关基因在肝脏中的异常表达。方法用原位杂交方法检测WHV/myc转基因小鼠肝肿瘤形成的不同阶段,c-myc转染基因、胰岛素样生长因子Ⅱ(IGF-Ⅱ)基因c-jun、c-fos和c-H-ras等基因的表达。结果c-myc转染基因和IGF-Ⅱ基因在转基因小鼠出生后早期的肝脏中异常表达,随后表达水平即降低至不能测出。上述两基因的表达在肝肿瘤形成期重新出现。c-jun、c-fos和c-H-ras基因在转基因小鼠肝癌发生前肝脏中的表达强度明显高于正常小鼠。结论c-myot染基因和IGF-Ⅱ基因在小鼠出生后早期和肿瘤形成期的异常表达对肝肿瘤的发生和瘤细胞转化表型的维持可能具有重要意义;在c-myc转染基因表达的“沉默”期,一些癌基因在肝脏中的异常表达对日后肝脏肿瘤的形成可能也具有一定作用。
Objective To detect the abnormal expression of related genes in the liver of WHV/myc transgenic mice. METHODS: Different stages of hepatic tumor formation in WHV/myc transgenic mice were detected by in situ hybridization. The c-myc transgene, IGF-II gene c-jun, c-fos and c-H- Expression of ras and other genes. Results The c-myc transfected gene and IGF-II gene were abnormally expressed in the early stage of the liver of the transgenic mice, and the subsequent expression level was reduced to be undetectable. The expression of the above two genes reappears in the liver tumor formation phase. The expression of c-jun, c-fos and c-H-ras genes in the liver of transgenic mice before liver cancer was significantly higher than that in normal mice. Conclusion The abnormal expression of c-myot and IGF-II in early postnatal and tumorigenesis phases may play an important role in the pathogenesis of hepatic tumor and the maintenance of the transforming phenotype of tumor cells. Gene expression in c-myc transfected cells may be important. In the “silent” period, abnormal expression of some oncogenes in the liver may also play a role in the formation of liver tumors in the future.