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目的:建立接骨挫伤胶囊中士的宁的含量测定方法。方法:采用高效液相色谱法,Diamonsil C18(250mm×4.6mm,5mm),流动相为乙腈-0.01mol/L庚烷磺酸钠与0.02mol/L磷酸二氢钾等量的混合液(用10%磷酸调节pH值2.8)(21∶79),流速1.0mL/min,柱温35℃,检测波长为254nm。结果:士的宁在0.01604~0.3028mg/mL范围内(进样量10μL)与峰面积呈良好的线性关系,平均加样回收率为99.06%、RSD=0.46%。结论:本方法简便、准确、重现性好,适用于该制剂的质量控制。
Objective: To establish a method for the determination of strychnine in the capsule of contusion and contusion. Methods: High performance liquid chromatography with Diamonsil C18 (250 mm × 4.6 mm, 5 mm) was used. The mobile phase consisted of a mixture of acetonitrile-0.01 mol / L sodium heptane sulfonate and 0.02 mol / L potassium dihydrogen phosphate 10% phosphoric acid to adjust pH 2.8) (21:79), flow rate 1.0mL / min, column temperature 35 ℃, detection wavelength 254nm. Results: The strychnine had a good linear relationship with the peak area in the range of 0.01604 ~ 0.3028mg / mL (injection volume 10μL). The average recoveries were 99.06%, RSD = 0.46%. Conclusion: The method is simple, accurate, reproducible and suitable for the quality control of the preparation.