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WRKY转录因子家族在长期的进化过程中,由低等到高等植物中发展和扩增.在植物应对干旱,低温和盐害等渗透非生物胁迫的响应中占据重要角色.本研究立足六倍体小麦基因组数据库,筛选获得一条新的WRKY转录因子Traes_ 3B_ 41047D5E6 (TaWRKY90),进一步克隆该转录因子全长序列,并通过荧光定量分析,构建进化树等相关方法验证了所筛选转录因子在植物对渗透胁迫相应中发挥作用.研究分析表明,所克隆TaWRKY90具有1个WRKY结构域,属于WRKY家族第 Ⅱ C亚族;其在不同时间的PEG-6000、低温、H2O2、ABA处理下,在小麦幼苗的根、叶片组织中均表现出不同程度的上调表达,且与MDA、CAT、SOD、POD四种酶的活性表达具有较高的相关性,表明TaWRKY90在小麦抗渗透胁迫的响应过程中发挥重要作用.“,”In the long evolution term,the WRKY transcription factor family has been developed and amplified from lower plant to higher plant,which plays an important role in the response to osmotic stress such as drought,low temperature and salt stress.In this experiment,we took the genome databases ofhexaploid wheat as the foundation to obtain a new WRKY transcription factor Traes 3B 41047D5E6 (TaWRKY90).The full-length sequence of this transcription factor was cloned and the quantitative analysis of fluorescence and the construction of phylogenetic tree were used to verify that the screened transcription factor play a role in the response to osmotic stress of plants.The results showed that the cloned TaWRKY90 possessed one WRKY structural domain,belonging to WRKY Ⅱ C subfamily.The expression of TaWRKY90 was up-regulated with different degrees in root and leaf of wheat under stress of PEG-6000,low temperature,H2O2 and ABA.The trend were similar to the variation trend of MDA,CAT,SOD,POD enzyme activity,which indicated that TaWRKY90 took a key role in the in the response of wheat to osmotic stress.