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经七步反应合成了Oxy-Cope重排反应的过渡态类似物-2-对羧丙氧基苯基环已醇(3),并以此为半抗原联接载体蛋白BSA制成抗原后免疫动物,从动物血清中分离纯化得到多克隆抗体。在200mmol/L的MES(4-吗啉乙磺酸)和200mmol/L的NaCl缓冲液中(pH=6.0),底物和抗体的摩尔比为100:1及37℃条件下,该抗体能催化3-对甲氧苯基-4-羟基-1,5-已二烯(1)重排生成6-对甲氧苯基-5-烯-已醛(2),v_(max)=15μmol/L·min,K_M=1025μmol/L,K_(cat.)=10min~(-1)。
The transition state analogue 2-p-carboxypropoxyphenylcyclohexanol (3) of the Oxy-Cope rearrangement reaction was synthesized in seven steps, and then the animals were immunized with the hapten-linked carrier protein BSA , Polyclonal antibody was isolated and purified from animal serum. The antibody can react with 200 mmol / L MES (4-morpholinoethanesulfonic acid) and 200 mmol / L NaCl buffer (pH = 6.0) at a substrate to antibody molar ratio of 100: 1 and 37 ° C The catalytic rearrangement of 3-p-methoxyphenyl-4-hydroxy-1,5-hexadiene (1) to 6-p-methoxyphenyl- /L·min, K_M = 1025μmol / L, K_(cat.)=10min~(-1).