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[目的]了解石家庄市男男性接触者(MSM)HIV-1感染者HIV-1毒株亚型类型,为制定MSM人群干预措施提供科学依据。[方法]提取36例HIV感染阳性者外周血单个核细胞(PBMC)的核酸,利用套氏PCR方法扩增env基因部分片断,并对扩增片断进行序列测定和分析。[结果]36例阳性者中21份毒株与国际标准株B亚型聚在一起,而与其他国际标准株远离,其组内基因距离为10.3±1.0;与国际标准株的基因距离为15.7±1.6,与其他亚型国际标准株间的基因距离均在25以上,亚型分类属于B亚型。14份毒株与国际标准参考株01-AE.TH.90.CM240 ACC U54771最近,基因距离为9.6±1.9,组内距离为5.6±0.9,属于CRF01-AE亚型。1份毒株与08-BC亚型国际参考株08BC.CN.97.97CNGX6F ACC AY008715聚在一起,基因距离为9.7±1.2,属于CRF08-BC亚型。[结论]HIV-1在石家庄市MSM中的流行株以B亚型和重组亚型CRF01-AE最常见,也存在CRF08-BC重组亚型。
[Objective] To understand the subtypes of HIV-1 strains in HIV-1 infected men who have sex with men (MSM) in Shijiazhuang and to provide a scientific basis for developing MSM population interventions. [Methods] Thirty - six HIV - infected peripheral blood mononuclear cells (PBMCs) were extracted from the peripheral blood mononuclear cells (PBMCs). Some of the env gene fragments were amplified by PCR, and the amplified fragments were sequenced and analyzed. [Results] Twenty-one of the 36 positive samples were clustered with the B subtype of the international standard strain, but far away from other international standard strains, and the gene distance within the group was 10.3 ± 1.0. The genetic distance to the international standard strain was 15.7 ± 1.6, and other subtype international standard strains were more than 25 genetic distances, subtypes belong to the B subtype. 14 strains of strains and the international reference strain 01-AE.TH.90.CM240 ACC U54771 recently, the genetic distance of 9.6 ± 1.9, intra-group distance of 5.6 ± 0.9, belonging to CRF01-AE subtype. One strain was clustered with the international reference strain 08BC.CN.97.97CNGX6F ACC AY008715 of 08-BC subtype, with a gene distance of 9.7 ± 1.2, belonging to the CRF08-BC subtype. [Conclusion] The epidemic strains of HIV-1 in Shijiazhuang MSM are the most common in subtype B and CRF01-AE, and the CRF08-BC subtype is also present.